Isolation of Plasma Kallikrein by High Efficiency Affinity Chromatography and Its Characterization.

نویسندگان
چکیده

برای دانلود باید عضویت طلایی داشته باشید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Preparation of Plasminogen by Affinity Chromatography

Background: Plasminogen is one of the compounds derived from human plasma. Activation of plasminogen produces plasmin. Plasmin is able to lyse fibrinogen, fibrin, and some other human plasma proteins. The aim of the present work was to study the separation of human plasminogen by affinity chromatography using gel lysine Sepharose. Materials and Methods: Normal human plasma was used as the...

متن کامل

Isolation of trypsins by affinity chromatography.

To learn the molecular mechanism of an enzyme action, first the protein has to be purified. Because of its simplicity and reproducibility, affinity chromatography has become a adopted method throughout the scientific community. Affinity chromatography occupies a unique place in separation technology since it is the only technique which enables purification of almost any biomolecules on the basi...

متن کامل

Vortex-Assisted Inverted Dispersive Liquid-Liquid Microextraction of Naproxen from Human Plasma and Its Determination by High-Performance Liquid Chromatography

Extraction and determination of naproxen from human plasma were performed using Vortex-Assisted Inverted Dispersive Liquid-Liquid MicroExtraction (VA−IDLLME) and High-Performance Liquid Chromatography (HPLC). The parameters affecting extraction recovery such as type and volume of extraction and disperser solvents, pH of sample solution, stirring rate, extraction time and salt addition were ...

متن کامل

Isolation and characterization of a digoxin-like immunoreactive substance from human urine by affinity chromatography.

A series of observations has suggested that one or more digoxin-like immunoreactive substances (DLIS) in biological fluids is able to cross-react with the antidigoxin antibody. Whether this substance is the endogenous inhibitor of Na+/K+ ATPase has not been well established. The aim of this study was to identify and characterize DLIS from human urine. Treated urine from healthy men was run on a...

متن کامل

Purification and characterization of soluble CD21 from human plasma by affinity chromatography and density gradient centrifugation.

Complement receptor II (CD21) is the receptor for C3d fragments on immune complexes. It also serves as a receptor for Epstein-Barr virus (EBV) and on B-lymphocytes CD21 amplifies signalling through the B cell receptor. CD21 is shed from the surface of the cell and is found circulating in plasma. There, soluble CD21 (sCD21) binds to CD23 and complement fragments, thereby modulating the immune re...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

ژورنال

عنوان ژورنال: Biological and Pharmaceutical Bulletin

سال: 2001

ISSN: 0918-6158,1347-5215

DOI: 10.1248/bpb.24.520